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primerscript reverse transcriptase kit  (TaKaRa)


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    Structured Review

    TaKaRa primerscript reverse transcriptase kit
    Primerscript Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primerscript+reverse+transcriptase+kit/pm39084096-60-1-14
    Average 86 stars, based on 1 article reviews
    primerscript reverse transcriptase kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Effects of miR-541 on neurite outgrowth during neuronal differentiation.
    Article Snippet: MicroRNA (miRNAs) are short non-coding RNA molecules that downregulate gene expression at post-transcriptional level. miRNAs are post-transcriptional regulators of gene expression important for neuron development and function.. This report demonstrated that a putative and chemically synthesized miRNA rno-mir-541 played an important role in the neuron development.. Differentiation of PC12 cells with nerve growth factor (NGF) is associated with neurite outgrowth, a process that involves upregulation of Synapsin I.

    Reverse Transcription:

    Article Title: Effects of miR-541 on neurite outgrowth during neuronal differentiation.
    Article Snippet: MicroRNA (miRNAs) are short non-coding RNA molecules that downregulate gene expression at post-transcriptional level. miRNAs are post-transcriptional regulators of gene expression important for neuron development and function.. This report demonstrated that a putative and chemically synthesized miRNA rno-mir-541 played an important role in the neuron development.. Differentiation of PC12 cells with nerve growth factor (NGF) is associated with neurite outgrowth, a process that involves upregulation of Synapsin I.

    Article Title: An effective homologous cloning method for isolating novel miR172s from Phalaenopsis hybrida
    Article Snippet: Total RNA was extracted from root, stem, leaf, pedicel, bud, sepal, petal, labellum and pistil of P. hybrida using RNAiso Plus (Takara). .. After treatment with DNase I, 1 μg of total RNA was used to synthesize first strand cDNA using a PrimerScript reverse transcriptase kit (Takara). ..

    Article Title: Transcriptomic profiling of human mesenchymal stem cells using a pulsed electromagnetic-wave motion bioreactor system for enhanced osteogenic commitment and therapeutic potentials.
    Article Snippet: Traditional bioreactor systems involve the use of three-dimensional (3D) scaffolds or stem cell aggregates, limiting the accessibility to the production of cell-secreted biomolecules.. Herein, we present the use a pulse electromagnetic fields (pEMFs)-assisted wave-motion bioreactor system for the dynamic and scalable culture of human bone marrow-derived mesenchymal stem cells (hBMSCs) with enhanced the secretion of various soluble factors with massive therapeutic potential.. The present study investigated the influence of dynamic pEMF (DpEMF) on the kinetic of hBMSCs.

    Article Title: Regulation of Seed Germination in the Close Arabidopsis Relative Lepidium sativum : A Global Tissue-Specific Transcript Analysis
    Article Snippet: .. One microgram of RNA was reverse transcribed using oligo(dT) primer according to the PrimerScript Reverse Transcriptase Kit instructions (TaKaRa). ..

    Article Title: Combination of azithromycin and methylprednisolone alleviates Mycoplasma pneumoniae induced pneumonia by regulating miR-499a-5p/STAT3 axis
    Article Snippet: Total RNA from clinical samples or A549 cells was extracted using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. After measuring the purity at a wavelength of 260 nm and concentration of RNA using NanoDrop 2000 (Thermo Fisher Scientific, Inc.), the extracted RNA was reversely transcribed into cDNAs using PrimerScript reverse transcriptase kit (Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol, followed by qPCR using SYBR Green kit (Qiagen GmbH) on an ABI7500 real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Article Title: E2F1 acts as a negative feedback regulator of c-Myc‑induced hTERT transcription during tumorigenesis.
    Article Snippet: .. For quantitative realtime PCR (qRT-PCR) analysis, total-RNA was extracted with TRIzol (Takara, Dalian, China), and cDNA was prepared using the PrimerScript reverse transcriptase kit (Takara). .. The mRNA expression levels of the targeted genes were analyzed by qRT-PCR using Applied Biosystems 7500 Real-Time PCR and SYBR-GreenERTM reagent system from Invitrogen.

    SYBR Green Assay:

    Article Title: Transcriptomic profiling of human mesenchymal stem cells using a pulsed electromagnetic-wave motion bioreactor system for enhanced osteogenic commitment and therapeutic potentials.
    Article Snippet: Traditional bioreactor systems involve the use of three-dimensional (3D) scaffolds or stem cell aggregates, limiting the accessibility to the production of cell-secreted biomolecules.. Herein, we present the use a pulse electromagnetic fields (pEMFs)-assisted wave-motion bioreactor system for the dynamic and scalable culture of human bone marrow-derived mesenchymal stem cells (hBMSCs) with enhanced the secretion of various soluble factors with massive therapeutic potential.. The present study investigated the influence of dynamic pEMF (DpEMF) on the kinetic of hBMSCs.

    Article Title: Combination of azithromycin and methylprednisolone alleviates Mycoplasma pneumoniae induced pneumonia by regulating miR-499a-5p/STAT3 axis
    Article Snippet: Total RNA from clinical samples or A549 cells was extracted using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. After measuring the purity at a wavelength of 260 nm and concentration of RNA using NanoDrop 2000 (Thermo Fisher Scientific, Inc.), the extracted RNA was reversely transcribed into cDNAs using PrimerScript reverse transcriptase kit (Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol, followed by qPCR using SYBR Green kit (Qiagen GmbH) on an ABI7500 real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Quantitative RT-PCR:

    Article Title: Transcriptomic profiling of human mesenchymal stem cells using a pulsed electromagnetic-wave motion bioreactor system for enhanced osteogenic commitment and therapeutic potentials.
    Article Snippet: Traditional bioreactor systems involve the use of three-dimensional (3D) scaffolds or stem cell aggregates, limiting the accessibility to the production of cell-secreted biomolecules.. Herein, we present the use a pulse electromagnetic fields (pEMFs)-assisted wave-motion bioreactor system for the dynamic and scalable culture of human bone marrow-derived mesenchymal stem cells (hBMSCs) with enhanced the secretion of various soluble factors with massive therapeutic potential.. The present study investigated the influence of dynamic pEMF (DpEMF) on the kinetic of hBMSCs.

    Real-time Polymerase Chain Reaction:

    Article Title: Co-transduction of ribosomal protein L23 enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in human gastric cancer.
    Article Snippet: .. For quantitative real-time PCR (qRT-PCR) analysis, total RNA was extracted with TRIzol, and cDNA was prepared using the PrimerScript reverse transcriptase kit (both from Takara Bio, Inc.). .. Expression levels of targeted genes were analyzed by qRT-PCR using Applied Biosystems 7500 Real-Time PCR and the SYBR®-GreenERTM reagent system from Invitrogen Life Technologies as described previously.

    Article Title: Combination of azithromycin and methylprednisolone alleviates Mycoplasma pneumoniae induced pneumonia by regulating miR-499a-5p/STAT3 axis
    Article Snippet: Total RNA from clinical samples or A549 cells was extracted using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. After measuring the purity at a wavelength of 260 nm and concentration of RNA using NanoDrop 2000 (Thermo Fisher Scientific, Inc.), the extracted RNA was reversely transcribed into cDNAs using PrimerScript reverse transcriptase kit (Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol, followed by qPCR using SYBR Green kit (Qiagen GmbH) on an ABI7500 real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Concentration Assay:

    Article Title: Combination of azithromycin and methylprednisolone alleviates Mycoplasma pneumoniae induced pneumonia by regulating miR-499a-5p/STAT3 axis
    Article Snippet: Total RNA from clinical samples or A549 cells was extracted using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). .. After measuring the purity at a wavelength of 260 nm and concentration of RNA using NanoDrop 2000 (Thermo Fisher Scientific, Inc.), the extracted RNA was reversely transcribed into cDNAs using PrimerScript reverse transcriptase kit (Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol, followed by qPCR using SYBR Green kit (Qiagen GmbH) on an ABI7500 real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.). ..

    Polymerase Chain Reaction:

    Article Title: E2F1 acts as a negative feedback regulator of c-Myc‑induced hTERT transcription during tumorigenesis.
    Article Snippet: .. For quantitative realtime PCR (qRT-PCR) analysis, total-RNA was extracted with TRIzol (Takara, Dalian, China), and cDNA was prepared using the PrimerScript reverse transcriptase kit (Takara). .. The mRNA expression levels of the targeted genes were analyzed by qRT-PCR using Applied Biosystems 7500 Real-Time PCR and SYBR-GreenERTM reagent system from Invitrogen.



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